雷米普利与BQ-123合用对大鼠在体心肌缺血／再灌注损伤的保护作用

目的：研究雷米普利与BQ-123合用对大鼠在体心肌缺血／再灌注损伤的影响。方法：健康雄性Wistar大鼠随机分为5组,制备缺血30min再灌注120min模型,采用雷米普利、BQ-123单用及两药联合应用的方式处理实验动物。观察两药合用对大鼠在体心肌缺血／再灌注损伤的保护作用。观察动物心率、血压、心电图ST-段变化,记录缺血期室性心律失常;检测血浆CK及LDH活力;心肌HE染色和TIC染色,定性和定量检测心肌梗死情况。结果：与I／R组比较,各给药组ST-段均明显降低;缺血期室性心律失常（VA）出现时间明显推迟且持续时间明显缩短,联合给药组作用更为显著;心律失常发生率明显降低;血浆CK及LDH活力显著降低且联合给药组降低更为显著;梗死面积明显缩小,心肌损伤程度明显减轻,其中联合给药组变化更为显著。结论：雷米普利、BQ-123单用及联合应用均对大鼠在体心肌缺血／再灌注损伤有保护作用,且两药合用在推迟缺血期VA的出现时间,缩短其持续时间,减少CK及LDH漏出,缩小心肌梗死面积方面优于两药各自单用。

Cardioprotection of ramipril and BQ-123 against myocardial ischemia/reperfusion injury in vivo in rats

Aim： To study the protective effects of ramipril in combination with BQ-123 on myocardial ischemia/reperfusion（I/R） injury in vivo in anesthetized rats. Methods： Healthy male Wistar rats were divided into 5 groups randomly and subjected to 30 min of myocardial ischemia followed by 120 min repeffnsion. Ramipril, BQ-123 and their combination were given to rats respectively. To observe the protection of their cornbination against rnyocardial I/R injury. HR, MAP and the change of ST-segment were observed. Ventricular arrthymias were monitored. The activity of creatine kinase（CK） and lactate dehydrogenase（LDH） in plasma, the infarct size and morphologic change were examined. Results： Compared with I/R group, the elevation of ST-segment was decreased. Onsets of VPC and VT were delayed, durations of VPC and VT were shortened, especially their combination. Incidences of VPC, VT and VF were decreased. Activity of plasma CK and LDH was decreased, especially their combination. IS, IS/AAR and the morphology of rnyocardium were improved, especially their combination. Condusion： Ramipril, BQ-123 and combined using these two agents protected rnyocardium from I/R injury in vivo. The protective effects on delaying onset of VA, shortening duration of VA, decreasing the activities of CK and LDH, decreasing infrarct size and improving morphology of myocardium were better than using ramipril and BQ-123 alone.