A parenchymatic medium solidifier for plant in vitro culture

A new material for the solidification of liquid culture media was prepared from plant parenchyma tissues by mechanical subdivision, solute extration and dessication from ethanol. It is suitable for in vitro culture and propagation of callus as well as shoot tip cultures. The following plant materials have been grown by means of the new medium solidifier: shoot cultures of Betula pendula Roth, Gerbera jamesonii H. Bolus ex Hook and Floribunda rose "Triumph", callus tissues of Daucus carota L. and Chenopodium album L. The new solidifying material has special advantages over agar for application in the rooting phase of in vitro propagation.Abbrevations PMS parenchymatic medium solidifier - MS Murashige and Scoog's medium - IAA Indole-3-acetic acid - B biotin - K kinetin - 2,4-D 2,4-dichlorophenoxyacetic acid - ch caseine hydrolysate