Antiviral activity of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP and ribavirin against Cauliflower mosaic virus in Brassica pekinensis |
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| Authors: | Josef ?pak Ivan Votruba Daniela Pavingerová Antonín Holy Vlastimila ?paková Karel Petrzik |
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| Affiliation: | 1. Institute of Plant Molecular Biology, Biology Centre of Academy of Sciences of the Czech Republic, v.v.i., Brani?ovsk?? 31, 370 05, ?esk?? Bud??jovice, Czech Republic
2. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i., Flemingovo n??m. 2, 166 10, Prague, Czech Republic
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| Abstract: | Antiviral effects of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP, and ribavirin on double-stranded DNA Cauliflower mosaic virus (CaMV) were evaluated in Brassica pekinensis plants grown in vitro on liquid medium. A double-antibody sandwich ELISA was used for relative quantification of viral protein and PCR for detection of CaMV nucleic acid in plants. Ribavirin and PMEA had no significant antiviral effect. (S)-HPMPC at concentration 50?mg?l?1 and PMEDAP at concentrations 50 and 12.5?mg?l?1 significantly (P??1 of (S)-HPMPC and PMEDAP. Reduction in fresh and dry weights of plants was significant (P??1 with both compounds. |
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