Insulin-like factor binding protein-3 promotes the G1 cell cycle arrest in several cancer cell lines |
| |
Authors: | Chen Wu Xiaobo Liu Yuanyuan Wang Huanna Tian Yuanyuan Xie Qinjian Li Xiaokang Zhang Fangming Liu |
| |
Affiliation: | 1. College of Life Sciences, Hebei University, Baoding, Hebei, 071002, PR China;2. College of Pharmaceutical Sciences, Hebei University, Baoding, Hebei, 071002, PR China |
| |
Abstract: | Insulin-like growth factor binding protein-3 (IGFBP-3) is a multi-functional protein known to induce apoptosis of various cancer cells in an insulin-like growth factor (IGF)-dependent and IGF-independent manner. In our previous study, we found that IGFBP-3 induced apoptosis through the activation of caspases in 786-O cells. In this study, we further examined that whether IGFBP-3 induced apoptosis through the induction of cell cycle arrest in 786-O, A549 and MCF-7 cells. Our results showed that overexpressed IGFBP-3 resulted in typical apoptotic ultrastructures in A549 cells under transmission electron microscope. The result of flow cytometry analysis indicated that IGFBP-3 arrested the cell cycle at G1-S phase in 786-O, A549 and MCF-7 cells. In A549 cells, quantitative real-time PCR and Western blot analysis showed a significant change in the expression of cell cycle-regulated proteins—a decrease in cyclin E1 expression, an increase in p21 expression. These results indicate a possible mechanism for G1 cell cycle arrest by IGFBP-3. Taken together, cyclin E1 and p21 may play important roles in the IGFBP-3-inducing G1 cell cycle arrest and apoptosis in several human cancer cells. |
| |
Keywords: | IGFBP-3, insulin-like growth factor binding protein-3 IGF, insulin-like growth factor IGFBPs, insulin-like growth factor binding proteins TNF-α, tumor necrosis factor-α RXRα, retinoid X rexeptorα CDK, cyclin-dependent kinase FCS, fetal calf serum TEM, transmission electron microscope examination PI, propidium iodide FITC, fluorescein isothiocyanate FACS, fluorescence-activated cell sorting qPCR, quantitative PCR GAPDH, glyceraldehyde 3-phosphate dehydrogenase TGF, transforming growth factor APC, anaphase promoting complex |
本文献已被 ScienceDirect 等数据库收录! |
|