The novel MER transposon-derived miRNAs in human genome |
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Authors: | Kung Ahn Jeong-An Gim Hong-Seok Ha Kyudong Han Heui-Soo Kim |
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Institution: | 1. Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 609‐735, Republic of Korea;2. Department of Genetics, Human Genetics Institute of New Jersey, Rutgers, the State University of New Jersey, 145 Bevier Rd, Piscataway, NJ 08854, USA;3. Department of Nanobiomedical Science & WCU Research Center, Dankook University, Cheonan 330‐714, Republic of Korea |
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Abstract: | MicroRNAs (miRNAs) are small RNA molecules (~ 20–30 nucleotides) that generally act in gene silencing and translational repression through the RNA interference pathway. They generally originate from intergenic genomic regions, but some are found in genomic regions that have been characterized such as introns, exons, and transposable elements (TE). To identify the miRNAs that are derived from palindromic MERs, we analyzed MER paralogs in human genome. The structures of the palindromic MERs were similar to the hairpin structure of miRNA in humans. Three miRNAs derived from MER96 located on chromosome 3, and MER91C paralogs located on chromosome 8 and chromosome 17 were identified in HeLa, HCT116, and HEK293 cell lines. The interactions between these MER-derived miRNAs and AGO1, AGO2, and AGO3 proteins were validated by immunoprecipitation assays. The data suggest that miRNAs derived from transposable elements could widely affect various target genes in the human genome. |
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Keywords: | TEs transposable elements MER MEdium Reiteration frequency AGO proteins argonaute proteins miRNA microRNA |
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