Isoenzyme patterns: a valuable molecular tool for the differentiation of Zygosaccharomyces species and detection of misidentified isolates |
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| Authors: | Duarte Filomena L Pais Célia Spencer-Martins Isabel Leão Cecília |
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| Institution: | aEstaçāo Vitivinícola Nacional, Instituto Nacional de Investigaçāo Agrária e das Pescas, Dois Portos, Portugal;bCentro de Biologia da Universidade do Minho (CBUM), Departamento de Biologia, Braga, Portugal;cCentro de Recursos Microbiológicos (CREM), Secção Autónoma de Biotecnologia, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal;dInstituto de Ciências da Vida e da Saúde, Universidade do Minho, Braga, Portugal |
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| Abstract: | Electrophoretic analysis of esterase, acid phosphatase, lactate dehydrogenase, glucose-6-phosphate dehydrogenase and alcohol dehydrogenase isoenzymes was performed in 39 strains classified into six species of the yeast genus Zygosaccharomyces. The electrophoretic profiles obtained allowed the clear separation of Z. bailii, Z. bisporus, Z. florentinus, Z. lentus, Z. mellis and Z. rouxii, strains of the latter species clustering into two subgroups. Furthermore, this methodology enabled the detection of misidentified strains, as subsequently confirmed by DNA-DNA reassociation and sequencing of the D1/D2 domain of the 26S rRNA gene. Cluster analysis of the global electrophoretic data and those obtained using only two of the isoenzyme systems, esterase and lactate dehydrogenase, yielded similar grouping of the strains examined, indicating that these enzymes are good markers for the differentiation of Zygosaccharomyces species. |
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| Keywords: | Zygosaccharomyces isoenzyme profiling yeasts |
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