The characteristics of a polygalacturonase produced by Bacillus polymyxa |
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| Affiliation: | 1. School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, Cambridge, MA 02138, USA;2. Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, 3335 Innovation Boulevard, Richland, WA 99354, USA;3. Biological Sciences Laboratory, Pacific Northwest National Laboratory, 902 Battelle Boulevard, Richland, WA 99354, USA;4. Department of Mineral Sciences, National Museum of Natural History, Smithsonian Institution, 10th St. & Constitution Ave. NW, Washington, DC 20560, USA;5. Department of Chemistry and Biochemistry, University of Oklahoma, 101 Stephenson Parkway, SLSRC, Room 1000, Norman, OK 73019, USA;6. Department of Earth Sciences, University of Minnesota, 310 Pillsbury Drive SE, Minneapolis, MN 55455, USA;7. Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, 266 Woods Hole Road, Woods Hole, MA 02543, USA;1. Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Carretera Ensenada-Tijuana 3918, Zona Playitas, 22860 Ensenada, B.C., Mexico;2. University of California, San Diego, Department of Chemistry and Biochemistry, 9500 Gilman Drive, La Jolla, CA 92093-0378, USA;1. School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, India;2. Department of Biotechnology, Harlal Institute of Management and Technology, Greater Noida, Uttar Pradesh, India;1. Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa;2. Department of Plant Science, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa;1. International Program in Hazardous Substance and Environmental Management, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand;2. Research Program in Hazardous Substance Management in the Agricultural Industry, Center of Excellences on Hazardous Substance Management (HSM), Bangkok 10330, Thailand;3. Department of Environmental Engineering, Faculty of Engineering, King Mongkut''s University of Technology, Thonburi 10140, Thailand;4. Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand;5. Center of Excellences on Hazardous Substance Management (HSM), Bangkok 10330, Thailand;6. Environmental Biotechnology Research Unit, Faculty of Environmental Management, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand |
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| Abstract: | The bacillus polygalacturonase (BPG) produced by Bacillus polymyxa was studied and characterized. Maximal cell yield and polygalacturonase production were obtained with a mineral medium containing biotin and 3% (w/v) pectin. Growth and BPG production are greatest when the culture is aerated on a rotary shaker.Pectinesterase production was dependent upon pectin concentration up to 4% BPG production is inducible. Pectin is the best inducer, followed by pectic acid and galacturonic acid in that order. The enzyme has an absolute requirement for calcium ion. Sodium, barium, manganese, strontium, zinc, ferric, cupric, and magnesium ions were ineffective in restoring the activity of dialyzed BPG. The pH optimum of BPG is between 8.9 and 9.4, and it is most stable between pH values of 5.0 and 8.0. Rapid loss of activity occurs below pH 5.0. Its temperature optimum is 45 °C. Although two separate enzyme fractions were isolated by cellulose column chromatography, both were similar in that they hydrolyzed pectic acid more rapidly than pectin. In addition it was shown that both fractions hydrolyzed pectic acid randomly. |
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