Studies on oxidative phosphorylation and ATPase activity of fresh and frozen brain mitochondria |
| |
| Affiliation: | 1. Division of Pharmacology, Faculty of Science, Utrecht University, Universiteitsweg 99, 3584 CG Utrecht, The Netherlands;2. Pharmacy Department, University College London Hospitals NHS Foundation Trust, London 2W1 2BU, United Kingdom;3. Department of Critical Care, University College London Hospitals NHS Foundation Trust, London NW1 2BU, United Kingdom;4. Bloomsbury Institute of Intensive Care Medicine, Division of Medicine, University College London, Gower Street, London WC1E 6BT, United Kingdom |
| |
| Abstract: | The presence of bovine serum albumin in the incubation medium resulted in increased P:O values with brain mitochondria in limiting concentrations as well as particles prepared in hypertonic sucrose or those exposed to a hypotonic medium.Frozen brain mitochondria retained some of their oxidative phosphorylating ability after storage for several days at −15 °C. The activity was greatly increased by the addition of DPN and nicoti namide to the incubation medium.The ATPase of freshly prepared brain mitochondria was almost completely devoid of 2,4-dinitrophenol-stimulating ability but was greatly activated by Mg++. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
