The action of synthetic surfactants on membranes of tumor cells: II. Titration experiments |
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| Affiliation: | 1. Université de Lyon, F-69008 Lyon, France;2. Université Lyon 1, Laboratoire d''Automatique et de Génie des Procédés, UMR CNRS 5007, F-69622 Villeurbanne, France;3. Université Lyon 1, Institut des Sciences Pharmaceutiques et Biologiques, Laboratoire de Dermopharmacie et Cosmétologie, F-69008 Lyon, France;4. Johnson & Johnson Santé Beauté France, Département des Nouvelles Technologies, Campus de Maigremont, F-27100 Val de Reuil, France;5. Université Lyon 1, Institut des Sciences Analytiques, UMR CNRS 5280, F-69622 Villeurbanne, France;1. School of Civil, Environmental and Architectural Engineering, Korea University, Seoul 136-701, Republic of Korea;2. Indian Institute of Technology Guwahati, Guwahati 781039, India;3. Department of Chemical Engineering, Institute of Chemical Technology, Mumbai 40019, India;1. Department of Electrical and Computer Engineering, New Jersey Institute of Technology, Newark, NJ 07102, USA;2. School of Applied Engr & Tech, New Jersey Institute of Technology, Newark, NJ 07102, USA |
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| Abstract: | A comparison has been made of the effect of increasing concentrations of a number of nonionic, anionic, and cationic surfactants on the cell membranes of the V-2 carcinoma, the Ehrlich ascites carcinoma and tissue culture preparations of human embryonic connective tissue and peripheral blood cells from human monocytic leukemia. The concentrations necessary to produce rupture of the cell membrane have been compared with those which promote uptake of the vital dye, nigrosin. The action of the agents in promoting staining and lysis has been roughly similar with the following exceptions: the V-2 cell membrane is more resistant to the action of SLS than the other cell lines, lauryl pyridinium chloride promotes staining but not lysis of the malignant and ascites lines, while the LLC-HE1 human connective tissue line is both stained and lysed in the presence of this surfactant.Within the range of 1 to 30 million cells, cell concentration is without much effect on the action of sodium lauryl sulfate against Ehrlich ascites cells. The presence of calcium chloride likewise does not cause much change in the action of this surfactant. Altering the pH from 1 to 9.4 causes little change in the uptake of dye by the cells.In general, when lysis does occur, it is preceded slightly by nigrosin staining. |
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