姬松茸在两种培养基上生长期间九种胞外酶活性变化 |
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引用本文: | 倪新江,丁立孝,潘迎捷,梁丽琨,由翠荣.姬松茸在两种培养基上生长期间九种胞外酶活性变化[J].菌物学报,2001,20(2):222-227. |
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作者姓名: | 倪新江 丁立孝 潘迎捷 梁丽琨 由翠荣 |
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作者单位: | 1. 烟台大学生化系 2. 莱阳农学院植保系 3. 上海农业科学院食用菌研究所 |
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基金项目: | 山东省教委资助项目!(J99I57) |
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摘 要: | 栽培在棉籽壳和麦草培养基上的姬松茸具有完整的胞外纤维素酶系;胞外CMC酶、FP酶、HC酶和蛋白酶活性呈周期性变化,其活性高峰对应于子实体的成熟期;胞外淀粉酶、漆酶和过氧化物酶在菌丝生长阶段活性较高,以后逐渐下降;胞外果胶酶和β-葡萄糖苷酶活性在整个栽培过程中变化不大;棉籽壳和麦草两种培养基中的九种胞外酶的活性变化趋势差异不大。
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关 键 词: | 姬松茸 胞外酶 活性变化 |
文章编号: | 1007-3515(2001)02-0222-0227 |
修稿时间: | 2000年9月8日 |
ACTIVITY CHANGES OF NINE EXTRACELLULAR ENZYMES FROM AGARICUS BLAZEI CULTIVATED ON COTTON SEED HULL AND WHEAT STRAW COMPOSTS |
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Authors: | NI Xin-Jiang DING Li-Xiao PAN Ying-Jie LIANG Li-Kun YOU Cui-Rong |
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Institution: | NI Xin-Jiang1 DING Li-Xiao2 PAN Ying-Jie3 LIANG Li-Kun1 YOU Cui-Rong1 |
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Abstract: | Agaricus blazei Murvill cultivated on cotton seed hull and wheatstraw composts had a complete set of extracellular cellulase. The activities of extracellular CMCase(carboxymenthel cellulase), FPase(filter paper cellulase), HCase (hemicellulase) and protease changed periodically and their peaks were all at the time when the fruitbody matured. The activities of extracellular amylase, laccase and peroxidase were comparatively high at mycelial growth stage, and gradually decreased afterwards. There were few changes in the activities of extracellular pectase and β- glucosidase in the whole period of cultivation. For the dynamic activity changes of the nine above extracellular enzymes, there was little difference between mycelium-colonised composts of cotton seed hull and wheat straw. |
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Keywords: | Agaricus blazei Extracellular enzyme Enzyme activity |
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