| 脑缺血损伤时脑片细胞内Ca2+及脂质过氧化物的测定 |
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| 引用本文： | 邢虹,何其华,袁兰,丁亚男,徐家鸰,吴本玠.脑缺血损伤时脑片细胞内Ca2+及脂质过氧化物的测定[J].生物物理学报,2000,16(4):813-818. |
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| 作者姓名： | 邢虹 何其华 袁兰 丁亚男 徐家鸰 吴本玠 |
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| 作者单位： | 1. 北京医科大学,生物物理学系,北京,100083
2. 北京医科大学,医药分析中心,北京,100083 |
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| 基金项目： | “九五”国家医学科技攻关项目!96 -906 -02 -21 |
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| 摘 要： | 用插细法制作局灶性脑缺血／再灌损伤模型，激光共聚焦扫描显微镜观察活体脑片细胞内Ｃａ＾２＋的分布及脂质过氧化物水平的动态变化，结果表明：缺血１ｈ时只有纹状体Ｃａ＾２＋含量增加，而缺血４ｈ时梗塞侧皮质、纹状体区域的Ｃａ＾２＋与脂质过氧化物含量均进一步增高，且与缺血时间相关，而与再灌持续时间无明显关系；轻度缺血／再灌损伤时细胞内脂质过氧化物增加的发生较增钙反应为早；纹状体对缺血／再灌损伤比皮质敏感。
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| 关 键 词： | 局灶性脑缺血 脂质过氧化物 钙离子 脑片细胞 |
MEASUREMENT OF INTRACELLULAR Ca2+ AND LIPID PEROXIDE IN BRAIN SLICES DURING CEREBRAL ISCHEMIA DAMAGE |
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| XING Hong,HE Qi-hua,YUAN Lan,DING Ya-nan,XU Jia-ling,WU Ben-jie.MEASUREMENT OF INTRACELLULAR Ca2+ AND LIPID PEROXIDE IN BRAIN SLICES DURING CEREBRAL ISCHEMIA DAMAGE[J].Acta Biophysica Sinica,2000,16(4):813-818. |
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| Authors: | XING Hong HE Qi-hua YUAN Lan DING Ya-nan XU Jia-ling WU Ben-jie |
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| Abstract: | The model of focal cerebral ischemia/reperfusion(I/R) was established in rats by reversibly inserting a nylon thread to observe dynamic changes of the intracellular Ca2 and lipid peroxide in brain slices using laser confocal scanning microscope. The results showed: At ischemia 1 hour the Ca2 was only increased in striatum,but at ischemia 4 hours the Ca2 and lipid peroxide markedly increased in infarcted lateral of cortex and striatum; The Ca2 and lipid peroxide were further increased at I/R, and being correlated to ischemia time, but not to reperfusion time; during slight I/R damage, the lipid peroxide increase appeared earlier than Ca2 ;The striatum was more sensitive to ischemia/reperfusion than cortex is. |
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| Keywords: | Laser Confocal Scanning Microscope Focal cerebral ischemia/reperfusion Lipid peroxide Calcium ion |
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