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Foot mucus is a good source for non-destructive genetic sampling in Polyplacophora |
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| Authors: | Amy N. S. Palmer Craig A. Styan Deborah C. A. Shearman |
| Affiliation: | (1) Centre for Research on the Ecological Impacts of Coastal Cities, University of Sydney, Sydney, NSW, 2006, Australia;(2) Present address: School of Life and Environmental Sciences, Deakin University, Warrnambool, Australia;(3) Fruit Fly Research Centre, University of Sydney, Sydney, Australia |
| Abstract: | A non-destructive method for collecting samples for DNA analysis from the mucus of molluscs was successfully adapted for use with the genus Ischnochiton. DNA was extracted using a Chelex-based method and the COI subunit of the mtDNA was amplified and sequenced. Sequences from the mucus were crosschecked against sequences from the foot tissue of the same animal and were found to be identical. This method provides a non-destructive way of carrying out larger studies of the genetics of rare organisms and may be of general use for genetic-based field studies of molluscs. |
| Keywords: | Non-destructive sampling Molluscs mtDNA Ischnochiton |
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