|
|||||
|
|
Effects of infection of EGFP-expressing Escherichia coli on haemocytes in Ciona intestinalis |
|
| Authors: | Liping Liu Changgong Wu Xiaojun Zhang Wen Luo |
| Affiliation: | a Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China b College of Aqua-Life Science and Technology, Shanghai Fisheries University, Shanghai 200090, China c Key Laboratory of Marine Ecology and Environmental Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China d Graduate School of the Chinese Academy of Sciences, Beijing, 100039, China |
| Abstract: | The effects of infection of EGFP-expressing Escherichia coli on the haemocytes of the ascidian Ciona intestinalis were investigated. The results showed that THC of the infected individuals changed significantly. Hyaline amoebocytes phagocytosed E. coli in 5 min and excreted lysosome particles that attached to the surface of the bacteria. Granular amoebocytes released lots of particles for humoral immunity while stem-cell-like haemocytes remained intact. With the increase in THC, the stem-cell-like haemocytes showed division and proliferation. A small portion of hyaline amoebocytes was at early apoptosis stage 1 h after infection and typical apoptosis bodies emerged in granular amoebocytes. A few of the infected haemocytes showed DNA damage using SCGE assay. Flow cytometry analysis revealed an obvious apoptosis peak in infected haemocytes. In conclusion, apoptosis was found to be an important immune response of ascidian haemocytes response to bacterial infection. To our best knowledge, this is the first report of the occurrence of apoptosis of haemocytes in ascidians. |
| Keywords: | EGFP, enhanced green fluorescence protein TUNEL, terminal-deoxynucleotidyl transferase mediated nick end labeling THC, total haemocytes count SCGE, single cell gel electrophoresis |
| 本文献已被 ScienceDirect 等数据库收录！ | |