Neonatal rat hepatocytes in primary tissue culture free from density-dependent regulation of growth |
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Authors: | Ubaldo Armato Paola G Andreis Enrica Draghi Loreta Mengato |
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Institution: | (1) Tissue Culture Laboratory, Department of Human Anatomy, Universisy Padova, I-35100 Padova, Italy |
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Abstract: | Summary Studies employing 3H]thymidine and radioautography as well as colchicine and Feulgen staining of DNA showed that up to 19-fold increases in the
degree of cell crowding in vitro, i.e. from 1.45 to 27.55×104 cells per specimen, did not change the rates of entry into DNA synthesis and mitosis of cultivated primary neonatal rat hepatocytes. |
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Keywords: | cultivated hepatocytes neonatal rat cell density contact inhibition growth regulation |
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