Capability of cultivated human hair-follicle cells to integrate with skin structure in vivo |
| |
Authors: | E S Chermnykh N V Radyukhina P N Rutkevich A Ya Shevelev T N Vlasik E A Vorotelyak A V Vasil’ev V V Terskikh |
| |
Institution: | 1.Koltsov Institute of Developmental Biology,Russian Academy of Sciences,Moscow,Russia;2.Institute of Experimental Cardiology, Cardiology Research-and-Production Complex,Federal Agency for Health Care and Social Development,Moscow,Russia |
| |
Abstract: | In the present work, we labeled human epidermal keratinocytes and dermal papilla cells in order to study their behavior after
intradermal transplantation. The cells were transduced by lentiviral vectors that bore a marker gene that encodes green fluorescent
protein (copGFP) or red fluorescent protein (DsRed). A portion of the transgene expressing cells was evaluated by flow cytometry.
The proposed genetic constructions have allowed one to achieve high efficiency (>95%) of the transduction of hair follicle
cells. The in vitro transduced cells were injected under epidermis of human skin fragments, after which these fragments were
transplanted under the skin of immunodeficient mice. The injected epidermal keratinocytes were found mainly in hair follicles
and partially in the zone of interfollicular epidermis, while dermal papilla cells were found in the papilla of the derma.
The results of the present study have shown that the chosen genetic constructions obtained based on human immunodeficiency
lentivirus are capable of the effective and stable transduction of human skin cells. The injected cells survived and were
found in the corresponding skin structures. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|