Characterization of two cDNAs for novel drought-inducible genes in the highly drought-tolerant cowpea |
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| Authors: | Satoshi Iuchi Kazuko Yamaguchi-Shinozaki Takeshi Urao Kazuo Shinozaki |
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| Institution: | (1) Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), Ministry of Agriculture, Forestry, and Fisheries, 2-1 Ohwashi, 305 Tsukuba, Ibaraki, Japan;(2) Laboratory of Plant Molecular Biology, The Institute of Physical and Chemical Research (RIKEN), Tsukuba Life Science Center, 3-1-1 Koyadai, 305 Tsukuba, Ibaraki, Japan;(3) Institute of Biological Science, Tsukuba University, Tennohdai, 305 Tsukuba, Ibaraki, Japan |
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| Abstract: | Ten cDNAs for drought-inducible genes were isolated using differential screening of a cDNA library prepared from 10-hr dehydrated
cowpea plants,Vigna unguiculata (S. Iuchi, K. Yamaguchi-Shinozaki, T. Urao, T. Terao, K. Shinozaki; Plant Cell Physiology, 1996 in press). Two of the cDNA
clones, designated CPRD12 and CPRD46, were sequenced and characterized. The CPRD12 and CPRD46 cDNAs encode putative proteins
related to nonmetallo-short-chain alcohol dehydrogenase (CPRD12) and chloroplastic lipoxygenase (CPRD46). Northern blot analysis
revealed that these genes are induced by high-salinity stress and exogenous abscisic acid, but not by cold stress. The CPRD46
gene is also responsive to heat stress and methyl jasmonate and salicylic acid. Genomic Southern blot analysis suggested that
CPRD12 constitutes a small gene family, but that CPRD46 is a single copy gene. We discuss the possible functions of these
two CPRD gene products under drought stress. |
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| Keywords: | Abscisic acid Cowpea (Vigna unguiculata) Drought Gene Expression Lipoxygenase Nonmetallo-short-chain alcohol dehydrogenase |
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