Evaluation of the phosphorescent palladium(II)-coproporphyrin labels in separation-free hybridization assays |
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Authors: | Burke Martina O'Sullivan Paul J Soini Aleksi E Berney Helen Papkovsky Dmitri B |
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Institution: | Biochemistry Department/ABCRF, University College Cork, Lee Maltings, Cork, Ireland. |
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Abstract: | Palladium(II)-coproporphyrin label and a set of corresponding monofunctional labeling reagents with different linker arms were evaluated for labeling of oligonucleotides and subsequent use in hybridization assays. The properties of resulting oligonucleotide probes including phosphorescence spectra, quantum yields, lifetimes, and labeling yields were examined as functions of the label and oligonucleotide structures. Upon hybridization with complementary sequences bearing dabcyl, QSY-7, and rhodamine green dyes, the probes displayed strong quenching due to close proximity effects. Intensity and lifetime changes of the phosphorescence, distance, and temperature dependences were investigated in detail. The potential of the new label and probes for sensitive and separation-free hybridization assays was discussed. |
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Keywords: | DNA probes Pd porphyrins Time-resolved fluorescence Hybridization assays Phosphorescence Proximity quenching |
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