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大鼠心肌肌浆网和核被膜ryanodine受体动力学特性的比较
引用本文:Wang PY,Yang J,Dong LW,Pang YZ,Tang CS. 大鼠心肌肌浆网和核被膜ryanodine受体动力学特性的比较[J]. 中国应用生理学杂志, 2002, 18(1): 43-46
作者姓名:Wang PY  Yang J  Dong LW  Pang YZ  Tang CS
作者单位:1. 第三军医大学病理生理教研室,重庆,400038
2. 北京大学第一医院心血管病研究所,北京,100034
基金项目:国家自然科学基金资助课题 ( 3970 0 2 0 0、39870 392 )
摘    要:
目的 :观察大鼠心肌浆网 (sarcoplasmicreticulum ,SR)和核被膜 (nuclearenvelope ,NE)ryanodine受体 (RyR)与配体结合特点及其蛋白质磷酸化调节。方法 :采用差速和等密度梯度离心分离心肌SR和NE ,用放射受体分析法研究RyR的特征。结果 :NE上存在高亲和力RyR ,其最大结合 (Bmax)为SRRyR的 1.7% ,解离常数 (Kd)为SR的6 0 %。分别用PKA和PKC磷酸化后 ,SR上该受体的Bmax各增加 3.7和 1.2倍 ,而NE上的该受体Bmax各增加 2 .2和 3.1倍 ,Kd均无显著改变。结论 :NE上存在比SR密度低但亲和力高的RyR ,能被PKA和PKC激活 ,而且对PKC较PKA更敏感

关 键 词:心肌  核被膜  肌浆网  ryanodine受体  磷酸化
文章编号:1000-6834(2002)01-0043-04
修稿时间:2000-01-10

Comparison of ryanodine binding to cardiac sarcoplasmic reticulum and nuclear envelope of rat
Wang Pei-Yong,Yang Jun,Dong Lin-Wang,Pang Yong-Zheng,Tang Chao-Shu. Comparison of ryanodine binding to cardiac sarcoplasmic reticulum and nuclear envelope of rat[J]. Chinese journal of applied physiology, 2002, 18(1): 43-46
Authors:Wang Pei-Yong  Yang Jun  Dong Lin-Wang  Pang Yong-Zheng  Tang Chao-Shu
Affiliation:WANG Pei-yong 2,YANG Jun 1,DONG Lin-wang 1,PANG Yong-zheng 1,TANG Chao-shu 1
Abstract:
Aim: The characteristics of ryanodine receptor in rat cardiac sarcoplasmic reticulum (SR) and nuclear envelope (NE) were studied. Methods: Velocity and isopyknic gradient centrifugation was employed to fractionate rat SR and NE. Ryanodine receptor was assayed with ryanodine saturate binding to the preparations. Results: The maximal binding (Bmax) and dissociating constant (Kd) of ryanodine receptor in rat cardiac NE were, 1.7% and 60% of those in SR respectively. Phosphorylation in vitro by PKA and PKC increased Bmax of the receptors in SR by 372% and 121%,and augmented those in NE by 221% and 306%, without any effects on Kd. Conclusion: Ryanodine receptors were present in rat myocardial NE,with lower density and higher affinity than those located in SR,which can be activated by PKA and PKC.
Keywords:myocardium  nuclear envelope  sarcoplasmic reticulum  ryanodine receptor  phosphorylation
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