Optical Biochip with Multichannels for Detecting Biotin–Streptavidin Based on Localized Surface Plasmon Resonance |
| |
Authors: | Shaoli Zhu Yongqi Fu |
| |
Institution: | (1) School of Electronic and Electrical Engineering, Nanyang Technological University, Singapore, Singapore;(2) School of Physical Electronics, University of Electronic Science and Technology of China, Chengdu, 610054, Sichuan Province, People’s Republic of China |
| |
Abstract: | A rapid and accurate detection of molecular binding of antigen-antibody signaling in high throughput is of great importance
for biosensing technology. We proposed a novel optical biochip with multichannels for the purpose of detection of biotin–streptavidin
on the basis of localized surface plasmon resonance. The optical biochip was fabricated using photolithography to form the
microarrays functioning with multichannels on glass substrate. There are different nanostructures in each microarray. Dry
etching and nanosphere lithography techniques were applied to fabricate Ag nanostructures such as hemispheres, nanocylindricals,
triangular, and rhombic nanostructures. We demonstrated that 100-nM target molecule (streptavidin) on these optical biochips
can be easily detected by a UV-visible spectrometer. It indicated that period and shape of the nanostructures significantly
affect the optical performance of the nanostructures with different shapes and geometrical parameters. Our experimental results
demonstrated that the optical biochips with the multichannels can detect the target molecule using the microarrays structured
with different shapes and periods simultaneously. Batch processing of immunoassay for different biomolecular through the different
channels embedded on the same chip can be realized accordingly. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|