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Isolation of the lipid-transporting protein Ns-LTP1 from seeds of the garden fennel flower (<Emphasis Type="Italic">Nigella sativa</Emphasis>)
Authors:Yu I Oshchepkova  O N Veshkurova  E A Rogozhin  A Kh Musolyamov  A N Smirnov  T I Odintsova  Ts A Egorov  E V Grishin  Sh I Salikhov
Institution:(1) Sadykov Institute of Bioorganic Chemistry, Academy of Sciences of the Uzbek Republic, ul. Mirzo-Ulugbeka 83, Tashkent, 100125, Uzbekistan;(2) Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia;(3) Russian State Agricultural University-Timiryazev Agricultural Academy, Moscow, Russia;(4) Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia
Abstract:A novel lipid-transporting protein (Ns-LTP1) has been isolated from seeds of the garden fennel flower Nigella sativa. The molecular mass, N-terminal amino acid sequence, and amino acid composition of the protein have been determined. Ns-LTP1 has a molecular mass of 9602 Da and contains eight cysteine residues which form four disulfide bridges. The protein is capable of suppressing the development of some phytopathogenic fungi and oomycetes.
Keywords:plants  Nigella sativa  antimicrobial peptides  lipid-transporting protein  antimicrobial activity  phytophthora infection
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