|
|||||
|
|
Molecular cloning and expression of a Bacillus subtilis β-glucanase gene in Escherichia coli |
|
| Authors: | BA Cantwell DJ McConnell |
| Institution: | 1. Arthur Guinness Son and Co. (Dublin) Ltd., St. James''s Gate, Dublin 8 Ireland Tel. 756701;2. Department of Genetics, Trinity College, Dublin 2 Ireland Tel. 772941 |
| Abstract: | A Bacillus subtilis gene coding for an endo-β-1,3-1,4-glucanase has been transferred to Escherichia coli by molecular cloning using bacteriophage λ and plasmid vectors. The gene is contained within a 1.6-kb EcoRI-PvuI DNA fragment and directs the synthesis in E. coli of a β-glucanase which specifically degrades barley glucan and lichenan. A novel dye-staining method has been developed to detect β-glucanase activity in colonies on agar plates. |
| Keywords: | Recombinant DNA barley glucan agar plate assay lambda phage plasmid vector pBR325 plasmid vector pAT153 maxicell technique Ap ampicillin Cm chloramphenicol CM-cellulose carboxymethylcellulose EtBr ethidium bromide G glucose kb kilobase pairs SDS sodium dodecyl sulphate Tc tetracycline |
| 本文献已被 ScienceDirect 等数据库收录！ | |