The Effect of TIP on Pneumovirus-Induced Pulmonary Edema in Mice |
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Authors: | Elske van den Berg Reinout A Bem Albert P Bos Rene Lutter Job B M van Woensel |
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Institution: | 1. Pediatric Intensive Care Unit, Emma Children''s Hospital, Academic Medical Center, Amsterdam, The Netherlands.; 2. Department of Respiratory Medicine and Experimental Immunology, Academic Medical Center, Amsterdam, The Netherlands.; The Ohio State University, United States of America, |
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Abstract: | BackgroundPulmonary edema plays a pivotal role in the pathophysiology of respiratory syncytial virus (RSV)-induced respiratory failure. In this study we determined whether treatment with TIP (AP301), a synthetic cyclic peptide that mimics the lectin-like domain of human TNF, decreases pulmonary edema in a mouse model of severe human RSV infection. TIP is currently undergoing clinical trials as a therapy for pulmonary permeability edema and has been shown to decrease pulmonary edema in different lung injury models.MethodsC57BL/6 mice were infected with pneumonia virus of mice (PVM) and received TIP or saline (control group) by intratracheal instillation on day five (early administration) or day seven (late administration) after infection. In a separate set of experiments the effect of multiple dose administration of TIP versus saline was tested. Pulmonary edema was determined by the lung wet-to-dry (W/D) weight ratio and was assessed at different time-points after the administration of TIP. Secondary outcomes included clinical scores and lung cellular response.ResultsTIP did not have an effect on pulmonary edema in different dose regimens at different time points during PVM infection. In addition, TIP administration did not affect clinical severity scores or lung cellular response.ConclusionIn this murine model of severe RSV infection TIP did not affect pulmonary edema nor course of disease. |
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