Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus
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| Authors: | Milan ?i?i? Miroslav ?ivi? Vuk Maksimovi? Marina Stani? Strahinja Kri?ak Tijana Cveti? Anti? Joanna Zakrzewska |
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| Institution: | 1. Institute for Multidisciplinary Research, Department of Life Sciences, University of Belgrade, Belgrade, Serbia.; 2. University of Belgrade-Faculty of Biology, Department of Physiology and Biophysics, Belgrade, Serbia.; 3. Institute of General and Physical Chemistry, NMR Laboratory, Belgrade, Serbia.; University of Nebraska-Lincoln, United States of America, |
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| Abstract: | The biological and chemical basis of vanadium action in fungi is relatively poorly understood. In the present study, we investigate the influence of vanadate (V5+) on phosphate metabolism of Phycomyces blakesleeanus. Addition of V5+ caused increase of sugar phosphates signal intensities in 31P NMR spectra in
vivo. HPLC analysis of mycelial phosphate extracts demonstrated increased concentrations of glucose 6 phosphate, fructose 6 phosphate, fructose 1, 6 phosphate and glucose 1 phosphate after V5+ treatment. Influence of V5+ on the levels of fructose 2, 6 phosphate, glucosamine 6 phosphate and glucose 1, 6 phosphate (HPLC), and polyphosphates, UDPG and ATP (31P NMR) was also established. Increase of sugar phosphates content was not observed after addition of vanadyl (V4+), indicating that only vanadate influences its metabolism. Obtained results from in
vivo experiments indicate catalytic/inhibitory vanadate action on enzymes involved in reactions of glycolysis and glycogenesis i.e., phosphoglucomutase, phosphofructokinase and glycogen phosphorylase in filamentous fungi. |
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