Presynaptic Modulation of K+-Evoked [3H]Dopamine Release in Striatal and Frontal Cortical Synaptosomes of Normotensive and Spontaneous-Hypertensive Rats |
| |
Authors: | Moulay A Yousfi-Alaoui Susanna Hospital Alicia Garcia-Sanz Albert Badia M Victoria Clos |
| |
Institution: | (1) Departament de Farmacologia i Terapèutica, Facultat de Medicina, Univeristat Autònoma de Barcelona, Spain |
| |
Abstract: | Regional differences in presynaptic 3H]dopamine (3H]DA) release and its modulation by D2 DA-receptors between the frontal cortex and striatum obtained from Wystar-Kyoto (WKY) and spontaneous-hypertensive rats (SHR) have been evaluated using superfused synaptosomes. Synaptosomal tritium content was significantly lower in the frontal cortex than in the striatum in both SHR and WKY (45% and 48%, respectively), but no differences in tritium content were obtained between strains. However, the 15 mM K+-evoked 3H]DA overflow was lower in the SHR as compared to WKY rats in both brain regions (striatum 23%, frontal cortex 21). Concentration-response curves for quinpirole (1nM-10 M)-mediated inhibition of 15mM K+-evoked 3H]DA release showed no differences between SHR and WKY. These results suggest that SHR has less ability to release 3H]DA as compared to WKY rats, but SHR did not show differences in the autoregulation of such release in both the frontal cortex and striatum. |
| |
Keywords: | Dopamine release hypertension striatum frontal cortex synaptosomes |
本文献已被 PubMed SpringerLink 等数据库收录! |