<Emphasis Type="Italic">In vitro</Emphasis> propagation of a high value medicinal plant: <Emphasis Type="Italic">Asparagus racemosus</Emphasis> Willd. |
| |
Authors: | Nishritha Bopana Sanjay Saxena |
| |
Institution: | (1) TERI University, Darbari Seth Block, Habitat Place, Lodhi Road, New Delhi, 110 003, India;(2) The Energy and Resources Institute (TERI), Darbari Seth Block, Habitat Place, Lodhi Road, New Delhi, 110 003, India |
| |
Abstract: | Asparagus racemosus Willd. is an important medicinal plant of tropical and subtropical India. Its medicinal usage has been reported in the Indian
and British Pharmacopoeias and in traditional systems of medicine such as Ayurveda, Unani, and Siddha. The multiple uses of
this species have increased its commercial demand, resulting in over-exploitation. Because of destructive harvesting, the
natural population of A. racemosus is rapidly disappearing, and it is recognized as ‘vulnerable’ (Warner et al., Some important medicinal plants of the Western
Ghats, India: a profile. International Development Research Centre, Artstock, New Delhi, India, 15 pp, 2001). The development of an efficient micropropagation protocol will play a significant role in meeting the requirements for
commercial cultivation, thereby conserving the species in its natural habitat. In the present study, in vitro shoot proliferation was obtained by culturing single node segments in Murashige and Skoog’s (MS) medium supplemented with
3.69 μM 2-isopentyl adenine and 3% sucrose with a multiplication rate of 3.5. For proper root formation, the in vitro-formed shoot clusters were cultured on half strength (major salts reduced to half) MS medium with 1.61 μM 1-naphthalene acetic
acid, 0.46 μM kinetin, 98.91 μM adenine sulfate, 500 mg/l malt extract, 198.25 μM phloroglucinol, and 3% sucrose. On this
medium, 85% rooting was observed within 20 d. Following a simple hardening procedure involving sequential transfer of plants
to a greenhouse, polyhouse, and shade net, the tissue-cultured plants were transferred to the field where the survival rate
was 100%. |
| |
Keywords: | Asparagus racemosus Willd Axillary branching Conservation Micropropagation |
本文献已被 SpringerLink 等数据库收录! |
|