MOC-31和CD44v6在良恶性腹水鉴别诊断中的应用

目的探讨检测肿瘤标志物MOC-31和CD44v6对鉴别良恶性腹水的诊断价值。方法利用液基薄层细胞学自动涂片技术方法筛查出查到肿瘤细胞的恶性腹水标本390例以及良性腹水标本100例,分别采用酶联免疫吸附法(ELISA)和免疫细胞化学染色检测MOC-31和CD44v6的含量和表达情况。结果 ELISA结果显示MOC-31和CD44v6在良性腹水中的含量分别为21±4ng/ml和291±32ng/ml,在恶性腹水中的含量分别为98.1±19.3ng/ml和891±116ng/ml,差异均具有显著性(P<0.05);免疫细胞化学染色显示MOC-31在恶性腹水细胞中阳性表达250例,良性腹水细胞中阳性表达5例;CD44v6在恶性腹水细胞中阳性表达266例,良性腹水细胞中阳性表达3例,差异均具有显著性(P<0.05)。结论 MOC-31和CD44v6可以做为良恶性腹水鉴别诊断的重要指标,值得在临床病理工作中推广应用。

MOC-31 and CD44v6 in the Differential Diagnosis of Benign Ascites and Malignant Ascites

Objective To investigate the diagnostic value to detect tumor markers MOC-31 and CD44v6 in differentiating benign from malignant ascites. Methods By thinprep cytology technique method , the ascites were screened to 390 cases malignant ascites specimens which include tumor cells and 100 cases benign ascites specimens, respectively. Enzyme-linked immunosorbent assay （ELISA） and immunocyto- chemistry staining were used to detect the content and expression of MOC-31 and CD44v6. Results The contents of MOC-31 and CD44v6 in the benign ascites were 21 ±4 ng / ml and 291 ±32 ng / ml, in malignant ascites were 98.1 ±19.3 ng / ml and 891 ± 116 ng / ml, the differences;were significant （P〈0. 05） ; MOC-31 positive expression in malignant ascites were 250 cases, therefore, the positive expression in benign ascites were 5 cases; CD44v6 positive expression in malignant ascites were 266 cases, however, the positive expression in benign ascites were 3 cases, the differences were statistically significant （P〈0.05）. Conclusion MOC-31 and CD44v6 can be used as an important indicator in the differential diagnosis of benign and malignant ascites, it was worthy promoting in clinical pathology work.