| 甲醇固定导致绿色荧光蛋白的荧光消失 |
| |
| 引用本文： | 李荣芳,袁慧,刘定干.甲醇固定导致绿色荧光蛋白的荧光消失[J].细胞生物学杂志,2007,29(2):303-304. |
| |
| 作者姓名： | 李荣芳 袁慧 刘定干 |
| |
| 作者单位： | 湖南农业大学动物科技学院，湖南农业大学动物科技学院，中国科学院上海生命科学研究院生物化学与细胞生物学研究所分子生物学国家重点实验室 长沙410128 中国科学院上海生命科学研究院生物化学与细胞生物学研究所分子生物学国家重点实验室，上海200031，长沙410128，上海200031 |
| |
| 摘 要： | 发现用甲醇固定转染了绿色荧光蛋白(GFP)基因的细胞会导致GFP的荧光消失,而当用聚甲醛固定时,GFP的荧光就没有失去。因此建议避免用甲醇对有GFP表达的细胞进行免疫组化前的固定。
|
| 关 键 词： | 甲醇 绿色荧光蛋白 |
| 修稿时间： | 2006-07-13 |
Fluorescence of Green Fluorescent Protein was Disappeared in Cells Fixed with Methanol |
| |
| Rong-Fang Li,Hui Yuan,Ding-Gan Liu.Fluorescence of Green Fluorescent Protein was Disappeared in Cells Fixed with Methanol[J].Chinese Journal of Cell Biology,2007,29(2):303-304. |
| |
| Authors: | Rong-Fang Li Hui Yuan Ding-Gan Liu |
| |
| Institution: | 1.College of Animal Medical, Hunan Agricultural University, Changsha 410128,China; 2.State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai 200031,China |
| |
| Abstract: | It was found that the fluorescence of GFP in cells transfected with green fluorescent protein (GFP) gene disappeared when those cells were fixed with methanol, whereas those fixed with paraformaldehyde remained fluorescent brightly. Therefore it is suggested that methanol be avoided in fixing cells expressing GFP for immunohistochemistry. |
| |
| Keywords: | methanol green fluorescent protein |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
