Purification and characterization of tubulin from the catfishHeteropneustes fossilis |
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Authors: | Anuradha Chaudhuri |
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Institution: | (1) Department of Biochemistry, Bose Institute, Centenary Building, 700 054 Calcutta, India |
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Abstract: | Tubulin was purified from the brain of the catfishHeteropneustes fossilis by cycles of temperature-dependent assembly and disassembly. Fish tubulin assembles into microtubules in the absence of high
molecular weight microtubule associated proteins. Its subunits comigrate with goat brain α andβ tubulin subunits and is composed of 4 major α andβ tubulins each as analyzed by isoelectric focusing and two dimensional gel electrophoresis. Peptide mapping showed it to be
very similar to goat brain tubulin. Polymerization of catfish brain tubulin occurs optimally between 18–37°C and the critical
protein concentrations of assembly at 18°C and 37°C are the same, as opposed to mammalian brain tubulins. |
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Keywords: | Tubulin Heteropneustes fossilis purification and properties |
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