生物酶/γ-Al2O3小球催化氧化柴油脱硫性能研究

通过吸附法将生物酶负载在γ-Al₂O₃小球载体上,并对生物酶/γ-Al₂O₃及载体进行扫描电镜(SEM)、比表面积分析(BET)、傅里叶红外光谱(FT-IR)及圆二色谱(CD)表征。结果表明:生物酶被吸附在载体上。将制备的生物酶/γ-Al₂O₃催化真实柴油氧化脱硫,考察了反应温度、反应流速和酶溶液浓度对真实柴油脱硫效果的影响,并对脱硫效果进行定性及定量分析;进一步对脱硫工艺条件进行响应面设计优化,找出最优反应条件。实验结果显示:反应温度49℃、反应流速1.0 mL/min、酶溶液浓度15.5%(酶载量为28.13 g),得出的最优脱硫率为93.16%;最后考察了该固定化酶的重复使用性能,该催化剂使用7次活性无明显降低,表明该固定化酶催化氧化柴油脱硫效果显著,具有潜在的工艺应用价值。

Study on the Performance of Oxidative Diesel Desulfurization Catalyzed by Bioenzyme/γ-Al2O3 Spheres

The bioenzymes were loaded on the γ-Al₂O₃ spherical carrier by adsorption method, and the bioenzymes/γ-Al₂O₃ and the carrier were characterized by scanning electron microscopy (SEM), specific surface area analysis (BET), Fourier infrared spectroscopy (FT-IR) and circular dichroism (CD). The results showed that the bioenzymes were adsorbed on the carriers. The prepared bioenzyme/γ-Al₂O₃ catalyzed oxidative desulfurization of real diesel fuel, and the effects of reaction temperature, reaction flow rate and enzyme solution concentration on the desulfurization effect of real diesel fuel were investigated, and qualitative and quantitative analyses of the desulfurization effect were performed; further response surface design optimization of the desulfurization process conditions was carried out to find out the optimal reaction conditions. The experimental results showed that the optimum desulfurization rate of 93.16% was derived from the reaction temperature of 49℃, reaction flow rate of 1.0 mL/min and enzyme solution concentration of 15.5% (enzyme loading of 28.13 g). Finally, the performance of the immobilized enzyme was investigated for repeated use, and the activity of the catalyst did not decrease significantly after seven uses, indicating that the immobilized enzyme is effective in catalyzing oxidative diesel desulfurization and has potential application value.