|
|||||
|
|
Regulation of cell proliferation and migration in gallbladder cancer by zinc finger X-chromosomal protein |
|
| Authors: | Zhujun Tan Shenglai Zhang Maolan Li Xiangsong WuHao Weng Qian DingYang Cao Runfa BaoYijun Shu Jiasheng MuQichen Ding Wenguang WuJiahua Yang Lin ZhangYingbin Liu |
| Institution: | Research Institute of Biliary Tract Disease, Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai, China; Laboratory of General Surgery and Department of General Surgery, Xinhua Hospital, Affiliated to Shanghai Jiao Tong University, School of Medicine, No. 1665 Kongjiang Road, Shanghai 200092, China |
| Abstract: | Gallbladder carcinoma (GBC) is one of the mostly aggressive and fatal malignancies. However, little is known about the oncogenic genes that contributed to the development of GBC. Zinc finger X-chromosomal protein (ZFX) was a novel member of the Krueppel C2H2-type zinc-finger protein family and its down-regulation led to impaired cell growth in human laryngeal squamous cell carcinoma. Here, we aim to investigate the function of ZFX in GBC cell proliferation and migration. Loss of function analysis was performed on GBC cell line (GBC-SD) using lentivirus-mediated siRNA against ZFX. The proliferation, in vitro tumorigenesis (colony-formation) ability as well as cell migration was significantly suppressed after GBC-SD cells which were infected with ZFX-siRNA-expressing lentivirus (Lv-shZFX). Our finding suggested that ZFX promoted the growth and migration of GBC cells and could present a potential molecular target for gene therapy of GBC. |
| Keywords: | GBC Gallbladder carcinoma ZFX Zinc finger X-chromosomal protein Lv-shZFX ZFX-siRNA-expressing lentivirus ZNF Zinc finger ZBTB20 Zinc finger and BTB domain containing 20 HCC Hepatocellular carcinoma ZNF267 Zinc finger protein 267 KCMF1 Potassium channel modulatory factor 1 siRNA Small interfering RNA DMEM Dulbecco's modified Eagle medium shRNAs Short hairpin RNAs EGFP Enhanced green fluorescent protein MOI Multiplicities of infection PCR Polymerase chain reaction MTT 3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyltetrazolium bromide FBS Fetal calf serum MMP Matrix metalloproteinase MMP-2 Matrix metallopeptidase 2 MMP-9 Matrix metallopeptidase 9 mRNA Messenger RNA |
| 本文献已被 ScienceDirect 等数据库收录！ | |