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Evaluation of reference genes for quantitative real-time RT-PCR analysis of gene expression in Nile tilapia (Oreochromis niloticus) |
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| Authors: | Chang Geng Yang Xian Li Wang Juan Tian Wei Liu Fan Wu Ming Jiang Hua Wen |
| Institution: | 1. Key Laboratory of Freshwater Biodiversity Conservation and Utilization of Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;2. Sarite Center for Stem Cell Engineering Translational Medicine, East Hospital, Stem Cell Research Center, Tongji University School of Medicine, Shanghai 200120, China |
| Abstract: | |
| Keywords: | GADPH glyceraldehyde-3-phosphate dehydrogenase UBCE ubiquitin-conjugating enzyme 18S rRNA 18S ribosomal RNA B2M beta-2-microglobulin EF1A elongation factor 1 alpha TUBA tubulin alpha chain-like ACTB beta actin PBS phosphate-buffered saline E PCR efficiency R2 correlation coefficients V pairwise variation LPS Lipopolysaccharide LTA lipoteichoic acid Ct cycle threshold CTSD cathepsin D CTSZ cathepsin Z NF sequential normalization factor |
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