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Delivery and processing of exogenous double-stranded DNA in mouse CD34 + hematopoietic progenitor cells and their cell cycle changes upon combined treatment with cyclophosphamide and double-stranded DNA |
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| Authors: | Evgenia V Dolgova Yaroslav R Efremov Konstantin E Orishchenko Oleg M Andrushkevich Ekaterina A Alyamkina Anastasia S Proskurina Sergey I Bayborodin Valeriy P Nikolin Nelly A Popova Elena R Chernykh Alexandr A Ostanin Oleg S Taranov Vladimir V Omigov Alexandra M Minkevich Vladimir A Rogachev Sergey S Bogachev Mikhail A Shurdov |
| Institution: | 1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630090, Russia;2. Novosibirsk State University, Novosibirsk 630090, Russia;3. Institute of Clinical Immunology, Siberian Branch, Russian Academy of Medical Sciences, Novosibirsk 630099, Russia;4. The State Research Center of Virology and Biotechnology VECTOR, Koltsovo, Novosibirsk Region 630559, Russia;5. LLC Panagen, Gorno-Altaisk 649000, Russia |
| Abstract: | We previously reported that fragments of exogenous double-stranded DNA can be internalized by mouse bone marrow cells without any transfection. Our present analysis shows that only 2% of bone marrow cells take up the fragments of extracellular exogenous DNA. Of these, ~ 45% of the cells correspond to CD34 + hematopoietic stem cells. Taking into account that CD34 + stem cells constituted 2.5% of the total cell population in the bone marrow samples analyzed, these data indicate that as much as 40% of CD34 + cells readily internalize fragments of extracellular exogenous DNA. This suggests that internalization of fragmented dsDNA is a general feature of poorly differentiated cells, in particular CD34 + bone marrow cells. |
| Keywords: | BMCs bone marrow cells CP cyclophosphamide DSB double-stranded break dsDNA double-stranded DNA HSCs hematopoietic stem cells ICL interstrand cross-link |
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