高转移鼠源性淋巴瘤细胞模型的建立及其生物学特性的研究

该文建立了高转移性小鼠淋巴癌细胞模型,并对其进行了生物学特性研究。LC1和LC2两个细胞系来自同一个小鼠淋巴瘤。通过细胞形态学观察,用常规的细胞生长曲线检测法、染色体核型分析、体外成球实验、侵袭和转移实验及体内成瘤实验检测细胞系的生物学特性及体内外致瘤能力。LC1细胞生长速度低于LC2细胞,LC1细胞的群体倍增时间为(24.33±2.12)h,LC2细胞的群体倍增时间为(20.52±2.71)h,差异显著。LC2与LC1比较,淋巴细胞髓系分化抗原Gr1表达量稍有增加,干细胞标记物Sca1和CD44表达均上调。LC1和LC2两个细胞系均为非整倍体核型,LC1和LC2细胞系非整倍体的比例分别为5.24%和89.12%。在不同的培养介质中LC2细胞系的克隆形成能力均比LC1强。LC2细胞系的迁移、侵袭能力及体内外致瘤能力明显比LC1强。该文发现,LC1细胞系在肾脏中出现少量转移灶,在肝脏中未发现转移灶,而LC2细胞系在肾脏中转移率为100%,在肝脏中也有大量转移灶出现。该研究成功地建立了高转移性小鼠淋巴癌细胞模型,为研究淋巴癌的高转移机制提供了良好的实验材料。

Establishment of a Highly Metastatic Murine Lymphoma Cell Model and Study on Their Biological Characteristics

This study established a highly metastatic murine lymphoma cell model.The malignant evolutionary cell subsets of mouse lymphoma cells were screened and their biological characteristics were studied.The biological characteristics and tumorigenicity of cell lines were examined by cell growth curve assay,chromosome karyotype analysis,spheroidization experiment in vitro,invasion and metastasis experiment and tumor formation experiment in vivo.The proliferative ability of LC2 was stronger than LC1,and the difference was significant.The population doubling time of LC1 cells was(24.33±2.12)h,and the population doubling time of LC2 cells was(20.52±2.71)h.Compared with LC1,the lymphocyte myeloid differentiation antigen marker Gr1 was slightly increased,and the expression levels of stem cell markers Sca1 and CD44 were up-regulated in LC2.Both LC1 and LC2 were aneuploid karyotypes,and the aneuploid ratios of LC1 and LC2 were 5.24%and 89.12%,respectively.The clone formation ability of LC2 was significantly stronger than that of LC1 in different culture media.The migration and invasion abilities of LC2 were also significantly stronger than those of LC1.The LC2 had stronger tumorigenic ability in vivo than the LC1.This study found that LC1 cell line had a small number of metastatic foci in the kidney,but had no metastasis foci in the liver,while LC2 cell line had a 100%metastasis rate in the kidney and a large number of metastatic foci in the liver.This study successfully established a high metastatic mouse lymphoma cell model,which provided a good experimental material for studying the mechanism of lymphatic cancer high metastasis.