| p300乙酰化在卡介苗感染中的作用 |
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| 引用本文： | 李苗,罗恩杰.p300乙酰化在卡介苗感染中的作用[J].微生物学杂志,2015(2):95-97. |
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| 作者姓名： | 李苗 罗恩杰 |
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| 作者单位： | 中国医科大学基础医学院 病原生物学教研室，辽宁 沈阳 110013;中国医科大学基础医学院 病原生物学教研室，辽宁 沈阳 110013 |
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| 基金项目： | 国家自然科学基金项目（81201333） |
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| 摘 要： | 研究p300乙酰化在卡介苗(bacillus Calmette Guérin，BCG)感染中的作用。构建THP-1巨噬细胞模型，比较BCG感染前后p300蛋白表达水平和组蛋白H3乙酰化水平的改变，加入p300特异性抑制剂Delphinidin，观察细胞内组蛋白H3乙酰化水平的变化。结果表明，在分化成熟的THP-1细胞系中，BCG感染能够上调p300蛋白表达水平和组蛋白H3乙酰化水平，加入p300特异性抑制剂Delphinidin后，组蛋白H3乙酰化水平降低。BCG感染通过p300途径导致蛋白质乙酰化水平发生改变。
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| 关 键 词： | 卡介苗（BCG） THP-1 p300 乙酰化 |
The Role of p300 Acetylation under BCG Infection |
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| LI Miao and LUO En jie.The Role of p300 Acetylation under BCG Infection[J].Journal of Microbiology,2015(2):95-97. |
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| Authors: | LI Miao and LUO En jie |
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| Institution: | Teach. & Res. Div. of Pathog. Biol., Coll. of Basic Med., China Med. Uni., Shenyang 110013;Teach. & Res. Div. of Pathog. Biol., Coll. of Basic Med., China Med. Uni., Shenyang 110013 |
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| Abstract: | The role of p300 acetylation of BCG (bacillus, Calmette Guérin) infection was studied. THP-1 macrophage model was constructed to compare the changes of the expression level of p300 protein and histone H3 acetylation level before and after BCG infection, adding p300 specific inhibitor of delphinidin, and observe the changes of acetylation level in H3 histone. The results showed that in a mature differentiation of THP-1 cell system, the BCG infection could up regulated the protein expression level of p300 and histone H3 acetylation level, and after adding p300 specific inhibition preparation of delphinidin, the level of histone H3 acetylation reduced. Therefore, BCG infection may lead to the change of protein acetylation level through p300 pathways. |
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| Keywords: | BCG THP-1 P300 acetylation |
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