| 镉胁迫对拟南芥的毒害作用及自噬现象的观测 |
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| 引用本文： | 高玲,张卫娜,陈文利.镉胁迫对拟南芥的毒害作用及自噬现象的观测[J].生物物理学报,2011(8). |
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| 作者姓名： | 高玲 张卫娜 陈文利 |
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| 作者单位： | 青岛农业大学生命科学学院;华南师范大学激光生命科学教育部重点实验室;广东省农业科学院;华南师范大学生命科学学院广东省植物发育生物工程重点实验室; |
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| 基金项目： | 教育部长江学者和创新团队发展计划项目(IRT0829); 广东省科技攻关项目(2007A020300008-6); 华南师范大学激光生命科学教育部重点实验室开放课题基金项目~~ |
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| 摘 要： | 镉离子(Cd2+)具有强植物毒性,可抑制植物生长,甚至导致植物死亡。为了研究重金属镉对拟南芥的毒害作用,采用叶绿素荧光技术、流式细胞技术、激光共聚焦技术及半定量RT-PCR技术,检测光合参数的变化、活性氧(reactive oxygen species,ROS)的累积、自噬的发生,以及病原相关蛋白(pathogenesis-related protein,PR)基因表达的变化。实验结果显示,随着50μmol/L CdCl2处理时间的延长,ROS和Cd2+在细胞中大量积累。而在镉胁迫的初期,会观察到自噬的发生及PR基因表达的变化。说明植物受到外界Cd2+作用的初期,会通过自噬及增强PR基因表达来抵抗外界胁迫。但随着处理时间的延长,植物细胞内累积了大量的ROS和Cd2+,当植物不足以通过自噬途径抵抗胁迫时,就会导致生长受阻,最终对光合系统造成损伤。
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| 关 键 词： | 镉 活性氧 自噬 叶绿素荧光 流式细胞技术 |
Cadmium Toxicity and Autophagy in Arabidopsis Seedlings |
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| GAO Ling,ZHANG Weina,CHEN Wenli, .College of Life Science,Qingdao Agricultural University,Qingdao,Sh,ong ,China,.MOE Key Laboratory of Laser Life Science,South China Normal University,Guangzhou ,.College of Life Science,Guangdong Key Lab of Biotechnology for Plant Development,.Guangdong Academy of Agricultural Sciences,Guangzhou.Cadmium Toxicity and Autophagy in Arabidopsis Seedlings[J].Acta Biophysica Sinica,2011(8). |
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| Authors: | GAO Ling ZHANG Weina CHEN Wenli College of Life Science Qingdao Agricultural University Qingdao Sh ong China MOE Key Laboratory of Laser Life Science South China Normal University Guangzhou College of Life Science Guangdong Key Lab of Biotechnology for Plant Development Guangdong Academy of Agricultural Sciences Guangzhou |
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| Institution: | GAO Ling1,2,ZHANG Weina2,4,CHEN Wenli2,3 1.College of Life Science,Qingdao Agricultural University,Qingdao,Shandong 266109,China,2.MOE Key Laboratory of Laser Life Science,South China Normal University,Guangzhou 510631,3.College of Life Science,Guangdong Key Lab of Biotechnology for Plant Development,4.Guangdong Academy of Agricultural Sciences,Guangzhou 510640 |
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| Abstract: | Cadmium(Cd),a strongly phytotoxic heavy metal,inhibits plant growth and even leads to plant death.In order to study the toxicity of Cd2+ to photosynthesis,accumulation of reactive oxygen species(ROS),autophagy and expression of pathogenesis-related protein(PR) were investigated using chlorophyll fluorescence,flow cytometry,laser confocal scan microscopy and semi-quantitative RT-PCR technology.Data showed that Cd2+ could result in accumulation of ROS and Cd2+.Autophagy and enhanced PR gene expression during ... |
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| Keywords: | Cadmium Reactive oxygen species Autophagy Chlorophyll fluorescence Flow cytometry |
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