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Sequence length and variation in the mitochondrial control region of two freshwater gobiid fishes belonging to Rhinogobius (Teleostei: Gobioidei)
Authors:I-S. Chen,&dagger  ,C.-H. Hsu,&dagger  ,C.-F. Hui,&Dagger  ,K.-T. Shao,&Dagger  ,P. J. Miller, L.-S. Fang,&dagger  
Affiliation:School of Biological Sciences, University of Bristol, Bristol, BS8 1UG, U.K.;Institute of Marine Resources, National Sun Yat-Sen University, Kaohsiung, Taiwan, R.O.C.;Institute of Zoology, Academia Sinica, Taipei, Taiwan, R.O.C.
Abstract:
The control region (D-loop) of mitochondrial DNA (mtDNA) was amplified and sequenced for eight samples of the rhinogobies Rhinogobius maculafasciatus and R. giurinus from Taiwan and southern China. The control regions of both species are of 841–842 bp; the length of these sequences being the most compact among all known sequences in teleost fishes. Three conserved sequence blocks (CSB) were observed. The full D-loop and tRNA Phe gene sequences were determined and compared with other fishes. The interspecific sequence divergence between the two species is 11.3–11.7%; and the intraspecific variation in R. guirinus 0.8–1.8%. Results suggest that the control region of Rhinogobius is informative for phylogenetic reconstruction at both intraspecific and interspecific levels in this gobiid genus.
Keywords:Rhinogobius    Gobiidae    mitochondrial genome    mtDNA sequence    D-loop    tRNA    polymerase chain reaction
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