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Steady-state kinetics of mouse DNA polymerase alpha |
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| Authors: | K Tanabe Y N Taguchi A Matsukage T Takahashi |
| Abstract: | Steady-state kinetic studies of DNA polymerase alpha purified from mouse myeloma MOPC104E cells have been carried out. The results of initial velocity analysis with or without sodium pyrophosphate, a product inhibitor, indicated that the reaction mechanism of this enzyme can be categorized as an ordered Bi Bi type where the concentration of the ternary complex is very low. |
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