Crystallization of fragment D from human fibrinogen. |
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Authors: | S J Everse H Pelletier and R F Doolittle |
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Institution: | Department of Chemistry, University of California at San Diego, La Jolla 92093-0634, USA. |
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Abstract: | Fragment D from human fibrinogen has been crystallized. The fragment, which is composed of three disulfide-linked chains (alpha' beta' gamma' = 88,000), was generated with either plasmin or mild trypsin digestion. The crystals diffracted out to 3.5 A; the space group is P2(1), unit cell dimensions a = 108 A, b = 48 A, c = 167 A, beta = 106 degrees. Fragment D was also co-crystallized with the ligand GPRP-amide, in which case the space group is consistent with P212121, unit cell dimensions a = 476 A, b = 82 A, c = 432 A. |
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Keywords: | crystallization fibrinogen fragment D GPRP-amide X-ray crystallography |
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