| WEB2基因参与酿酒酵母S期检查点调控 |
| |
| 引用本文： | 李新鸣,孙黎光,宣忠信,宋今丹. WEB2基因参与酿酒酵母S期检查点调控[J]. 细胞生物学杂志, 2002, 24(2): 121-124 |
| |
| 作者姓名： | 李新鸣 孙黎光 宣忠信 宋今丹 |
| |
| 作者单位： | [1]中国医科大学病原生物学教研室，沈阳110001 [2]中国中国医大生物化学与分子生物学教研室 |
| |
| 基金项目： | 国家自然科学基金,39870384 |
| |
| 摘 要： | WEB2基因编码产物为一种DNA解链酶。WEB2突变株经hydroxyurea阻断DNA合成后,经流式细胞仪检测DNA含量、纺锤体微管间接免疫 荧光染色及存活率测定,结果显示WEB2突变株表现S期检查点缺失。推测WEB2除了具有解链酶作用外,还参与酿酒酵母S期检查点调控机制,位于已建立的S期检查点信号传导通路模型上。本研究有助于加深对真核细胞检查点调控的了解,为研究肿瘤的发生机制提供线索。
|
| 关 键 词： | WEB2基因 酿酒酵母 S期检查点调控 |
| 修稿时间： | 2001-08-02 |
A ROLE OF WEB2 GENE IN S PHASE CHECKPOINT REGULATION OF S.CEREVISIAE |
| |
| LI Xin Ming SUN Li Guang XUAN Zhong Xin SONG Jin Dan. A ROLE OF WEB2 GENE IN S PHASE CHECKPOINT REGULATION OF S.CEREVISIAE[J]. Chinese Journal of Cell Biology, 2002, 24(2): 121-124 |
| |
| Authors: | LI Xin Ming SUN Li Guang XUAN Zhong Xin SONG Jin Dan |
| |
| Abstract: | WEB2 gene encodes a DNA helicase. A WEB2 mutant was treated with Hydroxyurea. Facs analysis, spindle microtubule staining by indirect immunofluorescence and % survial indicate that the mutant is defect in S phase checkpoint. We propose that WEB2 has not only helicase activity, but a role in S phase checkpoint regulation. And WEB2 gene locates in S phase checkpoint signal transduction pathway in S. cerevisiae. The study should shed light on eukaryotic cell checkpoint regulation and the origin of cancer. |
| |
| Keywords: | WEB2 gene S phase checkpoint S.cerevisiae |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
