| WEB2基因参与酿酒酵母S期检查点调控 |
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| 引用本文： | 李新鸣,孙黎光,宣忠信,宋今丹.WEB2基因参与酿酒酵母S期检查点调控[J].细胞生物学杂志,2002,24(2):121-124. |
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| 作者姓名： | 李新鸣 孙黎光 宣忠信 宋今丹 |
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| 作者单位： | [1]中国医科大学病原生物学教研室，沈阳110001 [2]中国中国医大生物化学与分子生物学教研室 |
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| 基金项目： | 国家自然科学基金,39870384 |
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| 摘 要： | WEB2基因编码产物为一种DNA解链酶。WEB2突变株经hydroxyurea阻断DNA合成后,经流式细胞仪检测DNA含量、纺锤体微管间接免疫 荧光染色及存活率测定,结果显示WEB2突变株表现S期检查点缺失。推测WEB2除了具有解链酶作用外,还参与酿酒酵母S期检查点调控机制,位于已建立的S期检查点信号传导通路模型上。本研究有助于加深对真核细胞检查点调控的了解,为研究肿瘤的发生机制提供线索。
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| 关 键 词： | WEB2基因 酿酒酵母 S期检查点调控 |
| 修稿时间： | 2001年8月2日 |
A ROLE OF WEB2 GENE IN S PHASE CHECKPOINT REGULATION OF S.CEREVISIAE |
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| LI Xin Ming SUN Li Guang XUAN Zhong Xin SONG Jin Dan.A ROLE OF WEB2 GENE IN S PHASE CHECKPOINT REGULATION OF S.CEREVISIAE[J].Chinese Journal of Cell Biology,2002,24(2):121-124. |
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| Authors: | LI Xin Ming SUN Li Guang XUAN Zhong Xin SONG Jin Dan |
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| Abstract: | WEB2 gene encodes a DNA helicase. A WEB2 mutant was treated with Hydroxyurea. Facs analysis, spindle microtubule staining by indirect immunofluorescence and % survial indicate that the mutant is defect in S phase checkpoint. We propose that WEB2 has not only helicase activity, but a role in S phase checkpoint regulation. And WEB2 gene locates in S phase checkpoint signal transduction pathway in S. cerevisiae. The study should shed light on eukaryotic cell checkpoint regulation and the origin of cancer. |
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| Keywords: | WEB2 gene S phase checkpoint S cerevisiae |
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