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Previously unclassified bacteria dominate during thermophilic and mesophilic anaerobic pre-treatment of primary sludge |
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| Authors: | Hasina M Pervin Damien J BatstonePhilip L Bond |
| Institution: | The University of Queensland, Advanced Water Management Centre (AWMC), St. Lucia, Brisbane, QLD 4072, Australia |
| Abstract: | Thermophilic biological pre-treatment enables enhanced anaerobic digestion for treatment of wastewater sludges but, at present, there is limited understanding of the hydrolytic–acidogenic microbial composition and its contribution to this process. In this study, the process was assessed by comparing the microbiology of thermophilic (50–65 °C) and mesophilic (35 °C) pre-treatment reactors treating primary sludge. |
| Keywords: | AD anaerobic digestion BLAST basic local alignment search tool FISH fluorescence in situ hybridisation HRT hydraulic retention time MP mesophilic pre-treatment OTU operational taxonomic unit RFLP restriction fragment length polymorphism TP thermophilic pre-treatment TPAD temperature phased anaerobic digestion T-RF terminal-restriction fragment T-RFLP terminal-restriction fragment length polymorphism |
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