Bradyrhizobium arachidis sp. nov., isolated from effective nodules of Arachis hypogaea grown in China |
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Authors: | Rui Wang Yue Li Chang Wen Tao Zheng Dan Zhang Xiao Xia Zhang Xin Hua Sui En Tao Wang Jia Qi Hu Li Ya Zhang Wen Xin Chen |
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Institution: | 1. State Key Laboratory for Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China;2. College of Lab Medicine, Hebei North University, Zhangjiakou 075000, China;3. Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China;4. Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 Mexico, DF, Mexico |
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Abstract: | Twenty-three bacterial strains isolated from root nodules of Arachis hypogaea and Lablab purpureus grown in five provinces of China were classified as a novel group within the genus Bradyrhizobium by analyses of PCR-based RFLP of the 16S rRNA gene and 16S–23S IGS. To determine their taxonomic position, four representative strains were further characterized. The comparative sequence analyses of 16S rRNA and six housekeeping genes clustered the four strains into a distinctive group closely related to the defined species Bradyrhizobium liaoningense, Bradyrhizobium yuanmingense, Bradyrhizobium huanghuaihaiense, Bradyrhizobium japonicum and Bradyrhizobium daqingense. The DNA–DNA relatedness between the reference strain of the novel group, CCBAU 051107T, and the corresponding type strains of the five mentioned species varied between 46.05% and 13.64%. The nodC and nifH genes of CCBAU 051107T were phylogenetically divergent from those of the reference strains for the related species. The four representative strains could nodulate with A. hypogaea and L. purpureus. In addition, some phenotypic features differentiated the novel group from the related species. Based on all the results, we propose a new species Bradyrhizobium arachidis sp. nov. and designate CCBAU 051107T (=CGMCC 1.12100T = HAMBI 3281T = LMG 26795T) as the type strain, which was isolated from a root nodule of A. hypogaea and had a DNA G + C mol% of 60.1 (Tm). |
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Keywords: | IGS 16S&ndash 23S rRNA intergenic spacer NJ neighbor-joining ML maximum likelihood MLSA multilocus sequence analysis RFLP restriction fragment length polymorphism |
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