Structure of neuromuscular junctions and differentiation of striated muscle fibers in mdx mice after bone-marrow stem cell therapy |
| |
Authors: | A V Sokolova V V Zenin V M Mikhailov |
| |
Institution: | 1.Institute of Cytology,Russian Academy of Sciences,St. Petersburg,Russia |
| |
Abstract: | Mdx mice are an experimental model of Duchenne muscular dystrophy caused by mutations in the dystrophin gene. Repeated cycles
of muscle degeneration-regeneration are common for mdx mice. Disrupted neuromuscular junctions also characterize mdx mice.
The structure of mdx mice neuromuscular junctions and the differentiation of striated muscle fibers were investigated 4, 8,
16, and 24 weeks after transplantation of C57BL/6 Lin(−) bone-marrow stem cells. We found that the death of striated muscle
fibers decreased 4 weeks after the transplantation of bone-marrow stem cells. Accumulation of muscle fibers without centrally
located nuclei began in 8 weeks and dystrophin synthesis increased in 16–24 weeks after the bone-marrow stem cells transplantation.
On the longitudinal sections of quadriceps muscle of mdx mice 4 weeks after transplantation, we observed a reduced quantity
of acetylcholine receptor clusters and an increase in their area in neuromuscular junctions. Sixteen weeks after the transplantation,
the total area of neuromuscular junctions increased due to an enlarged number of acethylcholine receptors and their extended
area. The single intramuscular transplantation of C57BL/6 Lin(−) bone-marrow stem cells induces the differentiation of mdx
mice striated muscle fibers and improves the structure of neuromuscular junctions. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|