Frankia菌种保藏

对用4种方法保藏的Frankia菌,进行了培养物存活、形态及其固氮活性的检测.发现在无氮液体培养基中保藏6年的Frankia.菌丝断裂,孢囊不完整.同期经有氮液体保藏的Frankia菌孢囊较完整.冷冻干燥保藏3.5年和砂管保藏8年,孢囊和菌丝均较完整.上述方法保藏的菌种,经活化后均能生长,且具有典型的Frankia菌形态特征和固氮活性.4种方法比较,无氮液体保藏法的菌体细胞生长速度快,固氮活性强,有侵染结瘤能力.

CONSERVATION OF FRANKIA CULTURE

Four conservation protocols of Frankia cultures were tested. Measured cell survival, morphology and nitrogen fixation activities of Frankia for each conservation protocol. Most sporangia and hypha were broken after storage in simple nitrogen-free liquid medium for 6 years. However, cultures stored in N-containing simple liquid medium for same time remained partially intact. Cultures stored in lyophilized milk for 3. 5 years or in sterile sand for 8 years were mostly intact. All the cultures mentioned above grew well and showed nitrogenase activity as well as typical morphological characteristic after an adaption period. Compared with other protocols, simple nitrogen-free liquid medium protocol were shown better in the item of cell growth, infectiveness and effectiveness.