赤霉酸产生菌UF456发酵条件的研究

筛选出赤霉酸产生菌UF456的发酵培养基配方,研究了各种碳源、氮源、碳氮比和不同微量元素对赤霉酸发酵的影响.结果表明:最佳碳源为玉米淀粉,含量为12.5%;最佳氮源为黄豆饼粉和花生饼粉,含量分别为0.3%和1.2%;微量元素Fe^(2+)、Cu^(2+)和Zn^(2+)对赤霉酸发酵具有促进作用,其中Fe^(2+)尤为重要,三种元素不同的组合对赤霉酸发酵的促进作用都具有累加效应.

STUDIES ON FERMENTATIVE CONDITIONS OF GIBBERELLIC ACID PRODUCED BY STRAIN UF456

Experiments were carried out on gibberellic acid fermentation conditions with its optimum medium formula. In gibberellic acid fermentation medium, the optimum carbon source was corn starch, and its optimum content was 12. 5 percent; the optimum nitrogen sources were peanut meal and soybean cakemeal, their optimum contents were 1. 2 and 0. 3 percent, respectively; the optimum carbon-nitrogen ratio was 50:1. The microelements of Fe, Cu and Zn could increase yield of gibberellic acid, and that Fe, as a single microelement, was more effective than others, and that they had accumulative effects on the gibberellic acid fermentation. Meanwhile, the effect of three microelements on the course of gibberellic acid fermentation was disscussed.