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Immobilization of levan fructotransferase for the production of di-fructose anhydride from levan |
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| Authors: | Seung Lim Ki-Young Lee Yong-Bok Lee Ki-Bang Song |
| Affiliation: | (1) Department of Material & Biochemical Engineering, Chonnam National University, Gwangju, 500-757, Korea;(2) Research Institute for Catalysis, Chonnam National University, Gwangju, 500-757, Korea;(3) Department of Pharmacy, Chonnam National University, Gwangju, 500-757, Korea;(4) Microbial Metabolic Engineering Research Unit and Genetic Resources Center, Korea Research Institute of Bioscience and Biotechnology, Taejon, 305-600, Korea |
| Abstract: | Levan fructotransferase (LFTase) from Arthrobacter ureafaciens K2032 was immobilized on various carriers of which Chitopearl BCW2501 beads showed the higher activity of 320 U g–1 for the formation of di-fructose anhydride compounds. The immobilized enzyme retained about 60% of its initial activity after being used for 20 cycles. |
| Keywords: | adsorption di-fructose anhydride immobilization ionic binding levan fructotransferase |
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