A room temperature ionic liquid as convenient solvent for the oxidative folding of conopeptides |
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| Authors: | Alesia A Miloslavina Enrico Leipold Michael Kijas Annegret Stark Stefan H Heinemann Diana Imhof |
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| Institution: | 1. Center for Molecular Biomedicine, Department of Biochemistry, Peptide Chemistry Group, Friedrich Schiller University Jena, Hans‐Kn?ll‐Strasse 2, D‐07745 Jena, Germany;2. Center for Molecular Biomedicine, Department of Biophysics, Friedrich Schiller University Jena, Hans‐Kn?ll‐Strasse 2, D‐07745 Jena, Germany;3. Institute of Environmental Chemistry and Technical Chemistry, Friedrich Schiller University Jena, Lessingstrasse 12, D‐07743 Jena, Germany |
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| Abstract: | We report the first example of conopeptide oxidation performed in a biocompatible ionic liquid, 1‐ethyl‐3‐methylimidazolium acetate (C2mim]OAc]), which enables the efficient formation of both hydrophilic and poorly water‐soluble conotoxins compared with conventional methods. Moreover, the method features a high‐concentration approach ultimately leading to higher yields at reduced separation effort. Copyright © 2008 European Peptide Society and John Wiley & Sons, Ltd. |
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| Keywords: | conotoxins cysteine‐rich peptides oxidative folding ionic liquids |
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