Halocyntin and papillosin,two new antimicrobial peptides isolated from hemocytes of the solitary tunicate,Halocynthia papillosa |
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| Authors: | Richard Galinier Emmanuel Roger Pierre‐Eric Sautiere Andre Aumelas Bernard Banaigs Guillaume Mitta |
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| Institution: | 1. Laboratoire de Biologie et d'Ecologie Tropicale et Méditerranéenne, CNRS‐UPVD‐EPHE, Perpignan, France;2. Laboratoire de Neurobiologie des Annélides, CNRS‐USTL, Lille, France;3. Centre de Biochimie Structurale, CNRS UMR5048;4. INSERM, U554;5. Université Montpellier 1 et 2, Montpellier, France;6. Laboratoire de chimie des Biomolécules et de l'Environnement, Université de Perpignan via Domitia, Perpignan, France |
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| Abstract: | We report here the screening of five marine invertebrate species from two taxa (tunicates and echinoderms) for the presence of cationic antimicrobial peptides (AMP) in defence cells (hemocytes). Antimicrobial activities were detected only in the two tunicates Microcosmus sabatieri and Halocynthia papillosa. In addition, we report the isolation and characterization of two novel peptides from H. papillosa hemocytes. These molecules display antibacterial activity against Gram‐positive and Gram‐negative bacteria. Complete peptide characterization was obtained by a combination of Edman degradation and mass spectrometry. The mature molecules, named halocyntin and papillosin, comprise 26 and 34 amino acid residues, respectively. Their primary structure display no significant similarities with previously described AMP. Copyright © 2009 European Peptide Society and John Wiley & Sons, Ltd. |
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| Keywords: | tunicate innate immunity antimicrobial peptide |
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