Synthesis of hepcidin derivatives in order to develop standards for immune adsorption method |
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| Authors: | Ádám Balogh Kata Horváti Gábor Mező László Derzbach Beáta Szebeni Lajos Nagy József Prechl Barna Vásárhelyi Ferenc Hudecz Szilvia Bősze |
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| Institution: | 1. First Department of Pediatrics, Semmelweis University, Budapest, Hungary;2. Research Group of Peptide Chemistry, Hungarian Academy of Sciences, L. E?tv?s University, Budapest, Hungary;3. Department of Applied Chemistry, University of Debrecen, Debrecen, Hungary;4. Department of Immunology, L. E?tv?s University, Budapest, Hungary;5. Research Group of Pediatrics and Nephrology, Hungarian Academy of Sciences, Budapest, Hungary;6. Department of Organic Chemistry, L. E?tv?s University, Budapest, Hungary |
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| Abstract: | MeCN, acetonitrile; ECL, enhanced chemiluminescence; EDT, 1,2‐ethanedithiole; HEPC12‐A, rabbit anti‐human hepcidin IgG, affinity purified; HEPC13‐A, rabbit anti‐mouse/human hepcidin IgG, affinity purified; HEPC61‐P, human hepcidin‐25 control/blocking synthetic peptide; HRP, horseradish peroxidase; IL‐6, interleukin‐6; KLH, keyhole limpet hemocyanin; LEAP, liver‐expressed antimicrobial peptide; NEM, N‐ethylmaleimide; NMP, N‐methyl‐pirrolidone; PBS, phosphate buffered saline; PVDF, polyvinylidene difluoride; SELDI‐TOF‐MS, surface‐enhanced laser desorption ionization–time‐of‐flight mass spectrometry; TMB, tetramethylbenzidin; TNF‐α, tumor necrosis factor‐α Copyright © 2009 European Peptide Society and John Wiley & Sons, Ltd. |
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| Keywords: | hepcidin hepcidin standards peptide synthesis immune adsorption method |
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