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Co-amplification of EBNA-1 and PyLT through dhfr-mediated gene amplification for improving foreign protein production in transient gene expression in CHO cells |
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| Authors: | Lee Joo-Hyoung Park Jong-Ho Park Sun-Hye Kim Sun-Hong Kim Jee Yon Min Jeong-Ki Lee Gyun Min Kim Yeon-Gu |
| Institution: | 1.Department of Biological Sciences, KAIST, 335 Gwahak-ro, Yuseong-gu, Daejeon, South Korea ;2.Biotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, South Korea ;3.Department of Bioprocess Engineering, KRIBB School of Biotechnology, Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, South Korea ;4.Department of Life Sciences, Imperial College London, South Kensington Campus, London SW7 2AZ, UK ;5.Department of Biomolecular Science, KRIBB School of Bioscience, Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, South Korea ; |
| Abstract: | Applied Microbiology and Biotechnology - Despite the relatively low transfection efficiency and low specific foreign protein productivity (qp) of Chinese hamster ovary (CHO) cell-based transient... |
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